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[Establishment and experimental study on mouse model of Chlamydia pneumoniae pneumonitis]
1Department of Respiratory Diseases, Nanjing Military General Hospital, Nanjing 210002, China.
Objective:
To evaluate mice as experimental animals for Chlamydia pneumoniae (C. Pneumoniae) infection and investigate the pathogenesis of C. pneumoniae pneumonitis.
Methods:
The Icr mice were inoculated with C. pneumoniae, strain CWL-029, by the intranasal or intravenous routes. After a single inoculation, mice were killed on the 1st, 3rd, 7th, 15th, 21th, 28th and 60th day separately. The pathological changes of lung tissue were analyzed.
Results:
The Icr mice were shown to be susceptible to C. pneumoniae. Inoculation of mice with C. pneumoniae induced a prolonged course of lung infection, as demonstrated by persistence of lung pathology (60 days). In the intranasal inoculation of mice, the lung pathology was characterized by patchy interstitial pneumonitis with predominately neutrophil leukocyte infiltration in the early(7 days) and lyphocytes infiltration in the later stages(14 days later) of infection. After i.v. inoculation, a similarly interstitial pneumonitis was seen, but it was milder and more patchy, especially in the early stage. C. pneumoniae DNA was detected by polymerase chain reaction(PCR) intermittently in the lung tissue. Inoculated mice developed serum IgG antibody responses.
Conclusions:
The Icr mice were shown to be susceptible to C. pneumoniae and the pulmonary infection was characterized by interstitial pneumonitis, especially in mice of intranasal inoculation.
Insights
Mice are susceptible to Chlamydia pneumoniae infection, developing prolonged lung inflammation characterized by interstitial pneumonitis, particularly after intranasal inoculation. This study validates mice as a model for C. pneumoniae research.
Area of Science:
- Microbiology
- Immunology
- Pathology
Context:
- Chlamydia pneumoniae is a significant human respiratory pathogen.
- Understanding its pathogenesis requires suitable animal models.
- Previous models have limitations in replicating human disease.
Purpose:
- To assess the utility of Icr mice as an experimental model for C. pneumoniae infection.
- To investigate the pathological mechanisms of C. pneumoniae-induced pneumonitis in mice.
Summary:
- Icr mice were intranasally or intravenously inoculated with C. pneumoniae strain CWL-029.
- Mice exhibited susceptibility, developing prolonged interstitial pneumonitis with distinct inflammatory cell infiltration patterns.
- C. pneumoniae DNA was intermittently detected, and serum IgG antibodies were induced.
Impact:
- Establishes Icr mice as a viable model for studying C. pneumoniae pathogenesis.
- Provides insights into the temporal dynamics of pulmonary inflammation in response to C. pneumoniae.
- Facilitates further research into C. pneumoniae-related respiratory diseases.