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Related Experiment Videos

Reproducible gene expression measurement among multiple laboratories obtained in a blinded study using standardized

E L Crawford1, G J Peters, P Noordhuis

  • 1Department of Medicine, Medical College of Ohio, Toledo, OH 43699, USA.

Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology
|January 5, 2002
PubMed
Summary

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Standardized RT (StaRT)-PCR demonstrates high interlaboratory reproducibility for gene expression analysis. This finding supports its use in developing large-scale gene expression databases for multi-institutional clinical studies.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Developing a gene expression database requires standardized, high-throughput, and cost-effective methods.
  • Standardized RT (StaRT)-PCR offers these advantages and has shown high intralaboratory reproducibility.

Purpose of the Study:

  • To assess the interlaboratory reproducibility of StaRT-PCR.

Main Methods:

  • A blinded, interlaboratory study was conducted.
  • Four laboratories measured the expression of ten genes using StaRT-PCR on a common complementary DNA sample.

Main Results:

  • The average coefficient of variation for nine quantifiable genes across laboratories was 0.48.
  • Expression of one gene was below the detection limit in all participating laboratories.

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Conclusions:

  • StaRT-PCR provides standardized, numerical, and reproducible data across multiple laboratories.
  • This reproducibility enables the development of a meaningful gene expression database for multi-institutional clinical studies.