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Updated: Jul 17, 2026
Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
Published on: November 7, 2018
Detection of hepatitis C virus RNA sequences in cholangiocarcinomas in Chinese and American patients
1Department of Pathology and Laboratory Medicine, School of Medicine, Tulane University, 1430 Tulane Avenue, New Orleans, Louisiana 70112, USA.
Objective:
To investigate the role of hepatitis C virus (HCV) in the malignant transformation of bile duct cells. Tissues from 6 Chinese patients and 6 American patients with cholangiocarcinoma were studied.
Methods:
RNA was extracted from the selected tumor areas of formalin-fixed, paraffin embedded sections, followed by reverse transcription double polymerase chain reaction (RT-PCR) and Southern blotting.
Results:
Positive and negative strand HCV RNA sequences were detected in seven out of twelve patients with cholangiocarcinoma. A high positive rate was found in Chinese patients (83%) as compared to US patients (33%).
Conclusion:
Our finding suggests HCV may play a role in the malignant transformation of bile duct cells.
