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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Directed biosynthesis of new enniatins
M Krause1, A Lindemann, M Glinski
1Chemisches Institut der Fakultät II, Fachrichtung Biochemie und Molekulare Biologie, Technische Universität Berlin, Germany.
The Journal of Antibiotics
|January 5, 2002
Summary
Researchers synthesized novel enniatins with potential anthelmintic properties using in vitro and in vivo methods. Modifications to amino acid and hydroxy acid residues created new enniatin variants with diverse functional groups.
Area of Science:
- Biochemistry
- Organic Chemistry
- Pharmacology
Background:
- Enniatins are cyclohexadepsipeptides with known biological activities.
- Developing new enniatin analogs is of interest for potential therapeutic applications.
Purpose of the Study:
- To produce novel enniatin derivatives with potential anthelmintic properties.
- To explore the impact of structural modifications on enniatin properties.
Main Methods:
- In vitro synthesis using enniatin synthetase.
- In vivo precursor feeding of Fusarium strains.
- Analysis by High-Performance Liquid Chromatography (HPLC), Nuclear Magnetic Resonance (NMR), and mass spectrometry.
Main Results:
- Successful production of new enniatin-type cyclohexadepsipeptides.
- Demonstrated ability to substitute N-methyl L-amino acid positions with various amino acids (e.g., alanine, cysteine, threonine, serine).
- Demonstrated ability to substitute D-2-hydroxyisovalerate residues with D-2-hydroxybutyric acid and D-lactic acid.
- In vitro synthesis yielded a broader product diversity compared to in vivo methods.
Conclusions:
- Both in vitro and in vivo strategies are effective for producing novel enniatins.
- Structural modifications allow for the generation of enniatins with varied functional groups and properties.
- The in vitro approach offers greater versatility in creating diverse enniatin analogs.
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