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[Direct chromosome analysis and FISH study of primary gastric cancer]
Objective:
To investigate chromosome aberrations and their role in the genesis and progression of primary gastric cancer.
Methods:
An improved, direct method of chromosome preparation from solid tumors was adopted for G-banding analysis followed by FISH on decolored G-banding chromosomes so that chromosome aberrations could be confirmed at DNA level.
Results:
A total of 28 primary gastric cancer specimens were studied. Case 1 and case 2 had simple chromosome numerical changes: 49, XY, +2, +8, +9 and 47, XX, +8, +20, respectively. All but case 1 and 2 had complicated chromosome abnormalities. Structural changes of frequent occurrence involved del(7q) (21/26), del(3p)(14/26), del(1p)(11/26) and del(17p)(10/26). The chromosome abnormalities could be simple or complicated. In the former, numerical changes involving 1 to 3 chromosomes could be observed. Trisomies 8 and 9 appeared to be a cytogenetic subgroup of primary gastric cancer. In the latter, del(7q) was the most consistent structural aberration. The 7q32-qter was the commonly lost segment.
Conclusion:
Numerical and structural alterations of chromosomes are present in primary gastric cancer. Del(7q) is one of the structural changes characteristic of primary gastric cancer. In the 7q32-qter segment, a tumor suppressor gene probably exists and it may have close relation to the genesis and progression of gastric cancer.