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[Detecting Epstein-Barr virus in nasopharyngeal carcinoma tissue using in situ polymerase chain reaction]
1Key Laboratory of Carcinogenesis of Chineses Ministry of Public Health, Hunan Medical University, Changsha, 410078, China.
Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology]
|January 5, 2002
Abstract
Objective:
To develop a sensitive non-isotopic method for in situ detection of Epstein-Barr virus (EBV) in paraffin-embedded section of nasopharyngeal carcinoma (NPC) tissue.
Methods:
The EBV BamHI-W fragment labeled with digoxigenin as probe was used to perform in situ hybridization and PCR in situ hybridization (in situ PCR).
Results:
The method of in situ PCR for the detection of EBV in NPC tissue was proved to be much more sensitive than in situ hybridization.
Conclusion:
Hybridization in situ of PCR product is useful to detect EBV in NPC tissues.