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Updated: Aug 4, 2026

Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells
Published on: August 27, 2019
[FHIT gene is abnormal in laryngeal carcinoma]
Objective:
To determine the role of FHIT gene in laryngeal carcinogenesis.
Methods:
Laryngeal cancer cell line Hep-2, HeLa cell line and 80 primary tumors from patients with laryngeal cancer were studied by means of RT-PCR, sequencing and microsatellite analysis.
Results:
Abnormal transcripts of FHIT gene were found in the two cell lines and 70% (14/20) of primary laryngeal carcinomas. Aberrant laryngeal tumor transcripts often lack more than two exons of FHIT gene. Microsatellite analysis showed that both loss of heterozygosity and microsatellite instability of FHIT gene existed in laryngeal carcinoma and the former was more common.
Conclusion:
FHIT gene participates in the laryngeal carcinogenesis and may be one of the candidate tumor suppressor genes.
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