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[Expression and characteristics of laryngeal carcinoma-associated antigen]
Objective:
To study the characteristics of laryngeal carcinoma-associated antigen (LCAA) and its expression on laryngeal carcinoma tissue.
Methods:
The binding of a cocktail monoclonal antibodies Lc9, Lc11, Lc12 to human laryngeal squamous-cell carcinoma, laryngeal precancerous lesions and normal laryngeal mucosa was examined by immunohistochemical method. LCAA isolated and purified by gel-filtration, and chromotography from 3 laryngeal squamous-cell carcinomas. The reactivity of the purified antigen was determined after treatment with trypsin, NaIO4, methanol and heating.
Results:
The positive rate of LCAA expression in 90 cases of laryngeal carcinoma, 14 cases of precancerous lesions and in 10 cases of normal laryngeal mucosa was 97.7%, 50.0% and 0, respectively. LCAA was a glycoprotein, moderately heat stable. The purified antigen gave two bands of apparent molecular weights of 61,400 and 56,500 on SDS-PAGE and Western blot.
Conclusion:
The results provide a basis for immunoimaging diagnosis and targeting chemotherapy for laryngeal cancer.