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Related Experiment Videos

RBC storage for 11 weeks.

J R Hess1, N Rugg, J K Gormas

  • 1Blood Research Detachment, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910-7500, USA. johnhess@his.com

Transfusion
|January 5, 2002
PubMed
Summary
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Extending red blood cell (RBC) storage to 11 weeks is achievable with experimental additive solutions (EASs), maintaining over 75% recovery and less than 1% hemolysis. This improves RBC availability and quality.

Area of Science:

  • Biomedical Science
  • Hematology
  • Blood Storage

Background:

  • Extending red blood cell (RBC) storage duration is crucial for enhancing RBC availability and quality.
  • This study investigates the efficacy of experimental additive solutions (EASs) for achieving 11-week RBC storage.

Purpose of the Study:

  • To evaluate the feasibility of storing RBCs for 11 weeks in EASs.
  • To assess the impact of mannitol concentration in EAS on RBC quality during extended storage.

Main Methods:

  • In vivo recovery of autologous RBCs stored for 11 weeks in EAS-67 was measured in volunteers.
  • RBCs were stored in EAS with varying mannitol concentrations (0-45 mmol/L) to assess hemolysis, morphology, and microvesicle formation.
  • RBC quality was monitored weekly during 12-week storage in EAS with 30 mmol/L mannitol.

Related Experiment Videos

Main Results:

  • RBCs stored for 11 weeks in EAS-67 showed 79% 24-hour in vivo recovery and 1.35% hemolysis.
  • Increased mannitol concentration reduced hemolysis but elevated microvesiculation.
  • EAS with 30 mmol/L mannitol enabled 11-week storage with hemolysis below 1% at both 11 and 12 weeks.

Conclusions:

  • Extended RBC storage up to 11 weeks is possible with EAS, achieving high recovery and low hemolysis.
  • Optimized EAS formulations, particularly with 30 mmol/L mannitol, support prolonged RBC viability and quality.