[In vivo microdialysis technique on rat myocardium]

The microdialysis procedure is a technique that has been established for some years. The heart, however, has several difficulties in the application of this technique. The heart is the beating in vivo, in contrast to other organs. I developed the flexibly mounted microdialysis technique, which involves the synchronized movement of the tip of the probe with the beating heart to reduce tissue injury. With this technique, it is feasible to make stable and long-term measurements of interstitial biological substances. By employing the flexibly mounted microdialysis technique, I will demonstrate that the monitoring of free radical generation and the level of adenosine measured during AMP perfusion that gives an index of the activity of ecto-5'-nucleotidase in the tissue. In the future, this technique will have versatile applications for useful studies to elucidate the actual mechanism in the pathogenesis of heart disorders.