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[Effect of IGF1 receptor gene antisense oligodeoxynucleotide on T24 urinary bladder cancer cells]
1Department of Urology, Shanghai First People's Hospital, Shanghai 200080, China.
Objective:
To evaluate the effects of autocrine blockage on T24 urinary bladder cancer cells.
Methods:
Semiquantitative RT-PCR, MTT determination, 3H thymidine incorporation, flow cytometry and electron microscopy were used to study the effects of antisense oligodexynucleotide (ODN) targeted against insulin-like growth factor 1 receptor (IGF1R) gene on IGF1R gene expression, drug sensitivity, proliferation and apoptosis of T24 cells.
Results:
After being treated with antisense ODN specific for IGF1R gene for 48 hours, the intrinsic IGF1R mRNA expression of T24 cells reduced approximately by 72.9%, which caused decrease in survival and increased sensitivity to mitomycin-induced apoptosis(P < 0.05).
Conclusion:
To block the autocrine loop with IGF1R antisense oligonucleotide may serve as a potential therapeutic approach to bladder cancer.
Insights
Blocking the insulin-like growth factor 1 receptor (IGF1R) with antisense oligonucleotide in T24 bladder cancer cells reduced IGF1R expression. This approach enhanced sensitivity to chemotherapy-induced apoptosis.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Context:
- T24 urinary bladder cancer cells exhibit an autocrine loop involving the insulin-like growth factor 1 receptor (IGF1R).
- Understanding the role of IGF1R in bladder cancer progression is crucial for developing targeted therapies.
Purpose:
- To investigate the therapeutic potential of blocking the autocrine IGF1R signaling pathway in T24 bladder cancer cells.
- To evaluate the impact of antisense oligodeoxynucleotide (ODN) targeting IGF1R on cancer cell behavior.
Summary:
- Treatment of T24 cells with an IGF1R-specific antisense ODN significantly reduced IGF1R mRNA expression by approximately 72.9% within 48 hours.
- This reduction in IGF1R expression led to decreased cell survival and enhanced sensitivity to mitomycin-induced apoptosis.
- Methodologies included semiquantitative RT-PCR, MTT assays, 3H thymidine incorporation, flow cytometry, and electron microscopy.
Impact:
- Autocrine IGF1R blockage via antisense oligonucleotide represents a promising therapeutic strategy for bladder cancer.
- Targeting the IGF1R pathway could offer a novel approach to overcome resistance and improve treatment outcomes in bladder cancer patients.