Related Experiment Videos
[Telomerase activity in rat tongue carcinogenesis]
1Department of Oral and Maxillofacial Surgery, Suzhou Stomatological Hospital, Suzhou, Jiangshu 215005, China.
Objective:
To detect the telomerase activity in rat tongue cancer and precancerous lesions and to investigate the possibility of telomerase as oral cancer biological marker.
Methods:
A total of 69 rat tongue carcinogenesis specimens induced by 4-nitroquinoline-1-oxide (4NQO) were examined for telomerase activity by PCR-telomeric repeat amplification protocol (TRAP) silver staining and ELISA.
Results:
Sequential increase in telomerase activity in tongue carcinogenesis was found. The positive rate of telomerase in normal, HP, mmDP, sDP, ISC and SCC was 0.0%, 12.5%, 25.0%, 66.7%, 72.7% and 93.8%.
Conclusions:
Telomerase may be a tumor specific gene marker, and may play an important role in early diagnosis and treatment of oral cancer.