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[Immunogenicity of phage-displayed tumor antigen-mimic peptide]
1Institute of Digestive Diseases, Xijing Hospital, 4th Military Medical University, Xi'an 710032, China.
Objective:
To determine the immunogenicity of phage-displayed tumor antigen-mimic peptide, and to lay experimental foundation for the development of gastric cancer vaccine.
Methods:
Based on previous studies, two phage-displayed peptide libraries were screened and 12 and 30 positive phage clones obtained from two libraries respectively. All the positive phages were purified and used them to immunize Balb/c mice. The immune serum was screened by immunohistochemical with tissue sections of human gastric cancer expressing MG7 antigen. The result was further confirmed by fluorescence labeling and ELISA.
Results:
Through immunohistochemical screening of immune sera with tissue sections of human gastric cancer, one phage displayed mimic peptide was able to elicit production of antibody specific for human gastric cancer.
Conclusion:
The result demonstrates that it is possible to develop tumor vaccine based on antigen epitope mimic peptide with immunogenicity.