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[Application of sequential culture in assisted reproductive technique]
1Center of Reproductive Medicine, Shandong Province Hospital, Jinan 250021, China.
Objective:
To investigate the effects of sequential culture technique on the outcomes of in vitro fertilization-embryo transfer(IVF-ET).
Methods:
Sequential culture media were used in 47 IVF-ET cycles, and conventional medium in 114 cycles. The fertilization rate, cleavage rate and speed, good embryo rate and pregnancy rate were compared between these 2 groups.
Results:
There were significantly higher embryo cleavage and quality score rate, pregnancy rate (97.9%, 64.4%, 46.7%) in sequential media group as compared those in conventional medium group (89.7%, 40.2%, 28.2%).
Conclusion:
Sequential culture system is more suitable for the development of cultured human oocytes and embryos.