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Updated: Aug 11, 2026

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
Published on: December 30, 2014
[Electrophoretic studies of dog's semen plasma in both fertile and sterile dogs]
Abstract:
Filter-paper electrophoretic partition of the semen plasma of fertile dogs showed the presence of three fractions. These fractions arranged themselves from the starting line solely in the direction of the negative pole; as compared with an electrophoregram of the dog blood serum, they placed themselves in the area of gamma-globulins. Electrophoretic partition of the semen plasma of dogs with fecundity disturbances also showed the presence of three fractions, whose migration path and protein concentration are identical with those found in the semen plasma of fertile dogs. It should be added that ejaculate samples obtained from old dogs with azoospermia did not partition into the fractions mentioned above. In such cases, only one fraction was observed at a certain distance from the starting line, becoming diffuse towards the cathode.
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