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Intracellular divalent cations and neuronal excitability.

K Krnjević, Y Lamour, J F MacDonald

    Canadian Journal of Physiology and Pharmacology
    |September 1, 1979
    PubMed
    Summary

    Intracellular magnesium (Mg) and cobalt (Co) injections in cat spinal motoneurones alter neuronal excitability and firing patterns. These divalent cations affect membrane potential, conductance, and afterhyperpolarizations, with Mg

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    Area of Science:

    • Neuroscience
    • Cellular Electrophysiology

    Background:

    • Divalent cations play crucial roles in neuronal function.
    • Understanding the specific effects of intracellular cations on motoneurone excitability is essential for deciphering neural control mechanisms.

    Purpose of the Study:

    • To investigate the intracellular effects of magnesium (Mg), manganese (Mn), cobalt (Co), and strontium (Sr) on cat spinal motoneurones.
    • To compare the actions of these divalent cations with calcium (Ca) and elucidate their impact on neuronal excitability and firing properties.

    Main Methods:

    • Intracellular injections of divalent cations (Mg, Mn, Co, Sr) into cat spinal motoneurones.
    • Recording of membrane potential, input conductance, action potentials, and postspike hyperpolarizing afterpotentials (a.h.p.).
    • Analysis of changes in neuronal excitability and current-firing relationships.

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    Main Results:

    • Intracellular Mg caused depolarization, decreased conductance, and depressed a.h.p., altering firing patterns.
    • Mn and Co also depressed a.h.p., with Co exhibiting a pronounced depolarizing action and strong depression of action potentials.
    • Intracellular Sr increased conductance, caused mild hyperpolarization, depressed excitatory postsynaptic potentials, and mimicked Ca effects.

    Conclusions:

    • Intracellular Mg, Mn, Co, and Sr exert distinct effects on spinal motoneurone electrophysiology.
    • Mg's effects are largely opposite to Ca, suggesting competitive interactions at K+ channels.
    • Sr shares similarities with Ca in its effects on motoneurones, particularly in depressing excitatory postsynaptic potentials.