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Molecular and physical characterization of Burkholderia mallei O antigens

Mary N Burtnick1, Paul J Brett, Donald E Woods

  • 1Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1.

Journal of Bacteriology
|January 16, 2002
PubMed

Insights

Burkholderia mallei lipopolysaccharide (LPS) differs from B. pseudomallei LPS. This study identifies the O-polysaccharide (O-PS) gene cluster and characterizes B. mallei O-PS structure.

Area of Science:

  • Microbiology
  • Immunology
  • Structural Biology

Background:

  • Burkholderia mallei lipopolysaccharide (LPS) exhibits cross-reactivity with polyclonal antibodies against B. pseudomallei LPS.
  • A specific monoclonal antibody (Pp-PS-W) targeting B. pseudomallei O-polysaccharide (O-PS) does not react with B. mallei LPS, suggesting structural differences.

Purpose of the Study:

  • To identify the O-polysaccharide (O-PS) biosynthetic gene cluster in Burkholderia mallei.
  • To characterize the molecular structure of the O-PS produced by B. mallei ATCC 23344.

Main Methods:

  • Identification of the O-PS biosynthetic gene cluster in B. mallei ATCC 23344.
  • Molecular characterization of the B. mallei O-PS structure.

Main Results:

  • The O-PS biosynthetic gene cluster of B. mallei ATCC 23344 was identified.
  • The molecular structure of the O-PS produced by B. mallei was characterized, revealing differences from B. pseudomallei O-PS.

Conclusions:

  • The distinct O-polysaccharide (O-PS) structure of Burkholderia mallei contributes to its differential reactivity with specific antibodies.
  • Understanding these structural variations is crucial for accurate serological differentiation between Burkholderia species.

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