Related Experiment Videos
Evidence for a fourth hydrogenase in Desulfovibrio fructosovorans
Laurence Casalot1, Gilles De Luca, Zorah Dermoun
1Laboratoire de Bioénergétique et Ingénierie des Protéines, UPR 9036-CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 09, France.
Journal of Bacteriology
|January 16, 2002
Abstract:
A strain devoid of the three hydrogenases characterized for Desulfovibrio fructosovorans was constructed using marker exchange mutagenesis. As expected, the H(2)-dependent methyl viologen reduction activity of the strain was null, but physiological studies showed no striking differences between the mutated and wild-type strains. The H(+)-D(2) exchange activity measured in the mutated strain indicates the presence of a fourth hydrogenase in D. fructosovorans.