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Related Experiment Videos

The MCS + revision A2 for highly efficient PBSC collection.

M Valbonesi1, P Carlier, A Bo

  • 1Immunohematology Service, San Martino University Hospital, Genova, Italy. robertabruni@smartino.ge.it

Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis
|January 17, 2002
PubMed
Summary

This study introduces an efficient protocol for collecting peripheral blood stem cells (PBSC), reducing procedure time and processing volume. The optimized method aims to improve stem cell collection efficiency for hemato-oncology patients.

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Area of Science:

  • Hematology
  • Oncology
  • Cellular Therapy

Background:

  • Autologous PBSC transplantation is crucial for hemato-oncology patients.
  • Improving the efficiency of PBSC collection is an ongoing research area.
  • Rejuvenation of apheresis systems aims to optimize cell collection.

Purpose of the Study:

  • To evaluate the efficiency of a new "version A2 protocol" for PBSC collection using an updated MCS+ apparatus.
  • To assess the impact of high blood flow rates and optimized recirculation on PBSC yield and procedure time.

Main Methods:

  • Implementation of the "version A2 protocol" with specific high blood flow rates for collection, reinfusion, and recirculation.
  • Performing 5 to 7 cycles with a single recirculation per 5 cycles.
  • Collection of PBSC from 27 patients, with 25 evaluable for efficiency.

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Main Results:

  • Achieved an average PBSC collection efficiency of 68.8%.
  • Average procedure time was 3.5-5 hours, processing 7,052 ml of blood.
  • Reduced RBC contamination to approximately 5.02 g hemoglobin and product volume to 177 ml.

Conclusions:

  • The "version A2 protocol" demonstrates high efficiency in PBSC collection.
  • This optimized protocol may reduce the gap between conventional (CFC) and definitive (DFC) PBSC collection outcomes.
  • Further confirmation is needed, but results suggest a significant improvement in PBSC collection technology.