Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lymphocyte adhesion to Candida albicans.

Christopher B Forsyth1, Herbert L Mathews

  • 1Department of Microbiology and Immunology, Loyola University of Chicago, Maywood, Illinois 60153, USA.

Infection and Immunity
|January 18, 2002
PubMed
Summary

Lymphocyte adherence to Candida albicans fungus is mediated by macrophage-1 antigen (Mac-1) (CD11b/CD18). This interaction is crucial for lymphocyte-driven antifungal effects against the fungus.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prebiotic intervention changes host and microbe proteomes in plasma extracellular vesicles of Parkinson's disease.

Scientific reports·2026
Same author

The Effect of a Race-Based Stress Reduction Intervention on Sleep Quality, Melatonin Onset, and Inflammation in African American Women: Protocol for a Pilot Randomized Controlled Trial.

Biological research for nursing·2026
Same author

Erratum: Light-dark shift promotes colon carcinogenesis through accelerated colon aging.

iScience·2025
Same author

Light-dark shift promotes colon carcinogenesis through accelerated colon aging.

iScience·2025
Same author

Gut microbiome dysbiosis is associated with lumbar degenerative spondylolisthesis in symptomatic patients.

JOR spine·2024
Same author

Effect of Alcohol on Clock Synchrony and Tissue Circadian Homeostasis in Mice.

Molecular nutrition & food research·2024

Area of Science:

  • Immunology
  • Mycology
  • Cell Biology

Background:

  • Lymphocyte adherence to Candida albicans is a critical initial step for direct antifungal immune responses.
  • Understanding the molecular mechanisms of this adherence is vital for developing novel antifungal therapies.

Purpose of the Study:

  • To identify the specific lymphocyte surface molecule responsible for adhesion to Candida albicans hyphae.
  • To elucidate the role of this molecule in mediating lymphocyte-induced antifungal activity.

Main Methods:

  • Utilized antibodies targeting macrophage-1 antigen (Mac-1) (CD11b/CD18) to block lymphocyte adhesion.
  • Tested the inhibitory effects of Mac-1 ligands (laminin, fibrinogen, RGD peptides, echistatin) and known Mac-1 inhibitors (N-Acetyl-D-glucosamine, beta-glucan).
  • Employed NIH 3T3 fibroblast transfectants expressing CD11b/CD18 to confirm Mac-1's role in binding.

Main Results:

  • Mac-1 (CD11b/CD18) was identified as the primary lymphocyte surface structure mediating adhesion to Candida albicans hyphae.
  • Antibodies against CD11b and CD18 subunits completely blocked lymphocyte adhesion.
  • Mac-1 ligands and known inhibitors significantly reduced lymphocyte adherence to the fungus.
  • Fibroblasts expressing CD11b/CD18 demonstrated binding to C. albicans, which was inhibited by anti-CD11b/CD18 antibodies.
  • Antibodies targeting Mac-1 effectively inhibited the antifungal activity of lymphocytes against C. albicans hyphae.

Conclusions:

  • Mac-1 (CD11b/CD18) is definitively identified as the key molecule mediating lymphocyte adhesion to Candida albicans hyphae.
  • This interaction is essential for the direct lymphocyte-mediated antifungal effect against C. albicans.
  • Targeting Mac-1 presents a potential strategy for enhancing antifungal immunity.

Related Experiment Videos