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[Insect juvenile hormone enhancing gene expression in silkworm baculovirus vector system]
H W Wang1, Z F Zhang, Q L Xiao
1Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|January 19, 2002
Summary
Juvenile hormone treatment enhances silkworm baculovirus expression. This boosts phytase and polyhedrin gene expression, increasing silkworm weight and survival time.
Area of Science:
- Biochemistry
- Entomology
- Molecular Biology
Background:
- Baculovirus expression systems are crucial for recombinant protein production.
- Silkworms (Bombyx mori) are utilized as hosts in these systems.
- Juvenile hormone (JH) plays a role in insect development and physiology.
Purpose of the Study:
- To investigate the effect of Juvenile hormone (JH) on the expression efficiency of phytase and polyhedrin genes in silkworms infected with recombinant baculovirus.
- To determine the impact of JH treatment on silkworm growth and survival rates.
Main Methods:
- 5th instar silkworms were infected with recombinant baculovirus (containing phytase gene) or wild-type BmNPV.
- Silkworms were treated with 100 ppm Juvenile hormone (JH) 48 hours post-ecdysis.
- Gene expression levels, LT50 (lethal time for 50% of the population), and average weight of infected silkworms were measured.
Main Results:
- Phytase and polyhedrin gene expression per silkworm increased by 30% and 40%, respectively, after JH treatment.
- The LT50 was extended by over 4 hours, indicating improved survival.
- Average weight of infected silkworms increased by 10%.
Conclusions:
- Juvenile hormone (JH) treatment significantly enhances the expression efficiency of phytase and polyhedrin genes in silkworms infected with baculovirus.
- The increased expression is attributed to prolonged virus replication time in JH-treated silkworms.
- JH treatment positively impacts silkworm health, leading to increased weight and survival.