The mitochondrial complex I: progress in understanding of catalytic properties
Related Experiment Video
Updated: Aug 14, 2026

Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution
Published on: June 1, 2017
A D Vinogradov1, V G Grivennikova
1Department of Biochemistry, School of Biology, Moscow State University, Russian Federation. adv@biochem.bio.msu.su
Abstract:
Although the mitochondrial Complex I has been extensively studied for over 4 decades, its catalytic properties in mitochondria are poorly understood. This review summarizes the data on standard and nonstandard kinetic parameters of the enzyme. The slow interconversion between active and deactivated forms of the mammalian Complex I (A/D transition) is described. We discuss the potential relevance of this transition for the regulation of NADH oxidation by the respiratory chain under physiological and pathophysiological conditions.
Related Concept Videos
The Inner Mitochondrial Membrane
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
ATP Synthase: Mechanism
The Supercomplexes in the Crista Membrane
Translocation of Proteins into the Mitochondria
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Electron Transport Chain: Complex III and IV

