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Published on: October 25, 2016
[Molecular cloning of the tumor associated antigen recognized by monoclonal antibody 3H11]
1Beijing Institute for Cancer Research, Peking University, Beijing 100034, China.
Objective:
Monoclonal antibody (McAb) 3H11 can specifically bind to different cancer cells from different tissues. Meanwhile, McAb 3H11 labeled with radioactive isotopes has been successfully applied to detect primary cancer and metastatic cancer in clinical imaging. Molecular cloning of the antigen recognized by McAb 3H11 will be significant for understanding tumor development.
Methods:
Based on the fragment of the McAb 3H11 antigen cloned by Immunoscreening MGC803 cDNA expression library, the whole length of the cDNA molecule was gotten by RACE and nested PCR.
Results:
Sequence data of the cDNA molecule indicates that there was no homologous gene in GenBank. Northern blot experiments showed that mRNA of McAb 3H11 antigen extensively distributes in different cancer cells and tissues but not in corresponding normal tissues. Moreover in producing antibodies of the antigen expressed prokaryotically, it was found that the immunogenicity of the antigen was quite low in mammalian.
Conclusion:
The antigen recognized by McAb 3H11 acts as a regulator in embryo cells and regains expression in tumor cells; and it may be associated with low differentiation and high proliferation.
Insights
Researchers identified a novel cancer antigen recognized by monoclonal antibody (McAb) 3H11. This antigen is expressed in tumors but not normal tissues, suggesting its role in cancer development and potential for targeted therapies.
Area of Science:
- Oncology
- Molecular Biology
- Immunology
Context:
- Monoclonal antibody (McAb) 3H11 demonstrates specific binding to diverse cancer cells.
- Radioisotope-labeled McAb 3H11 is effective in clinical imaging for detecting primary and metastatic cancers.
Purpose:
- To achieve molecular cloning of the antigen recognized by McAb 3H11.
- To understand the antigen's role in tumor development.
Summary:
- The full-length cDNA of the McAb 3H11 antigen was obtained using RACE and nested PCR, based on an initial fragment identified via Immunoscreening.
- Sequence analysis revealed no homologous genes in GenBank.
- Northern blot confirmed extensive mRNA distribution in various cancer cells and tissues, with absent expression in corresponding normal tissues.
- Prokaryotic expression of the antigen yielded low immunogenicity in mammalian systems.
Impact:
- The cloned antigen is identified as a potential regulator in embryonic cells, with re-expression observed in tumor cells.
- The antigen's expression pattern suggests a correlation with low differentiation and high proliferation rates in cancers.
- This discovery provides a molecular target for understanding tumor development and may inform future cancer diagnostics and therapeutics.
