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Updated: Jul 31, 2026
Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury
Published on: February 23, 2014
Olfactory epithelium grafts in the cerebral cortex: an immunohistochemical analysis
E H Holbrook1, L J DiNardo, R M Costanzo
1Department of Otolaryngology-Head and Neck Surgery, Virginia Commonwealth University, Medical College of Virginia Campus, Richmond, Virginia 23298-0551, USA.
Objective:
To develop an alternative model for studying the regenerative capacity of olfactory neurons.
Study Design:
An immunohistochemical analysis of mouse olfactory epithelium transplanted to the cerebral cortex.
Methods:
Strips of olfactory epithelium removed from donor mice at postnatal day 5 to day 20 were inserted into the parietal cortex of adult mice. Recipient animals were allowed to survive for 25 to 120 days and then perfused with 4% paraformaldehyde 1 hour after bromodeoxyuridine injection. The brains were processed, and frozen sections were obtained. Sections through transplant tissue were analyzed using immunohistochemistry and compared with normal olfactory epithelium.
Results:
Graft survival approached 85% with mature olfactory neurons detected in 35% of the transplants stained for olfactory marker protein. Transplant epithelium resembled normal olfactory epithelium containing mature olfactory neurons and axon bundles.
Conclusions:
Studies of olfactory neuron regeneration have been limited by the inability to produce cultures with long-term viability. Olfactory epithelial grafts to the cerebral cortex provide an alternative approach to the study of olfactory neuron regeneration.
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