Related Experiment Video
Updated: Aug 12, 2026
Method of Direct Segmental Intra-hepatic Delivery Using a Rat Liver Hilar Clamp Model
Published on: April 2, 2017
Protective effect of low dose of ascorbic acid on hepatobiliary function in hepatic ischemia/reperfusion in rats
1College of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Changan-gu, Suwon 440-746, South Korea.
Background/Aims:
The aim of this study was to investigate the effect of ascorbic acid (AA) an alterations in hepatic secretory and microsomal functions during hepatic ischemia and reperfusion.
Methods:
Rats were subjected to 60 min of hepatic ischemia, and 1 and 5 h of reperfusion. Five minutes prior to ischemia, the animals were administered either vehicle or ascorbic acid (AA) (30, 100, 300, and 1000 mg/kg) intravenously.
Results:
The serum aminotransferase level and lipid peroxidation were markedly higher as a result of ischemia/reperfusion. These increases were significantly attenuated by AA doses of 30 and 100 mg/kg but were augmented by dose of 1000 mg/kg. Bile flow and cholate output were markedly decreased by ischemia/reperfusion. AA doses of 30 and 100 mg/kg restored but dose of 1000 mg/kg inhibited their secretion. Both the cytochrome P(450) content and aminopyrine N-demethylase activity were decreased by ischemia/reperfusion, which were prevented by AA doses of 30 and 100 mg/kg but were aggravated by dose of 1000 mg/kg. Aniline p-hydroxylase activity was elevated by ischemia/reperfusion, and this was prevented by AA doses of 100, 300 and 1000 mg/kg.
Conclusions:
Ischemia/reperfusion diminishes the hepatic secretory and microsomal functions. AA has both antioxidant and pro-oxidant effects, depending upon the dose.
