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Leakage evaluation of root end filling materials using endotoxin
Hong Ming Tang1, Mahmoud Torabinejad, James D Kettering
1Loma Linda University, School of Dentistry, CA 92350, USA.
Journal of Endodontics
|January 25, 2002
Summary
Mineral trioxide aggregate (MTA) demonstrated superior sealing ability by significantly reducing endotoxin leakage compared to other dental materials over 12 weeks. This finding highlights MTA
Area of Science:
- Endodontics
- Dental Materials Science
- Bacteriology
Background:
- Endotoxin, derived from Gram-negative bacteria, is implicated in periradicular lesion development.
- Effective sealing of root canals is crucial to prevent bacterial ingress and associated pathologies.
- Mineral trioxide aggregate (MTA) is recognized for its sealing properties.
Purpose of the Study:
- To compare the endotoxin-sealing efficacy of Mineral trioxide aggregate (MTA) against Super-EBA, IRM, and amalgam.
- To evaluate the long-term sealing performance of these dental materials using endotoxin as a tracer.
Main Methods:
- A modified Limulus Amebocyte Lysate test was employed to quantify endotoxin leakage.
- Four dental materials (MTA, Super-EBA, IRM, amalgam) were tested for their ability to prevent endotoxin passage.
- Leakage was assessed at multiple time points: 1, 2, 6, and 12 weeks.
Main Results:
- Mineral trioxide aggregate (MTA) exhibited significantly less endotoxin leakage compared to IRM and amalgam at all tested time points (p < 0.05).
- MTA demonstrated significantly lower endotoxin leakage than Super-EBA at 2 and 12 weeks (p < 0.05).
Conclusions:
- Mineral trioxide aggregate (MTA) provides superior long-term sealing against endotoxin penetration compared to IRM, amalgam, and Super-EBA.
- The findings support the use of MTA as a reliable material for obturation in endodontic procedures requiring excellent coronal seal.