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Periapical lesion progression with controlled microbial inoculation in a type I diabetic mouse model
Ashraf Fouad1, Jody Barry, Jessica Russo
1Department of Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington 06030-1715, USA.
Journal of Endodontics
|January 25, 2002
Summary
Poorly controlled diabetes mellitus increases mortality risk from periapical infections in nonobese diabetic (NOD) mice. Polymerase chain reaction (PCR) proved more sensitive than culturing for detecting bacteria in these infections.
Area of Science:
- Oral Biology
- Infectious Diseases
- Endocrinology
Background:
- Diabetes mellitus is linked to severe periapical infections.
- Understanding diabetes' impact on periapical pathogenesis is crucial for infection control.
Purpose of the Study:
- To investigate how diabetes mellitus affects periapical lesion development.
- To evaluate microbiological techniques for detecting persistent bacteria in diabetic mice.
Main Methods:
- Periapical lesions were induced in nonobese diabetic (NOD) and control mice.
- Exposed pulps were inoculated with bacteria or oral flora.
- Bacterial presence was assessed using culturing and PCR.
- Lesion size and interleukin-6 levels were measured.
Main Results:
- NOD mice exhibited significantly higher mortality rates compared to controls, especially with inoculated exposures.
- Chronic uninoculated NOD mice showed significant weight loss.
- PCR demonstrated higher sensitivity than culturing for detecting anaerobic bacteria.
- Lesion size and IL-6 levels were comparable between NOD and control mice.
Conclusions:
- Diabetes mellitus exacerbates mortality from periapical infections.
- PCR is a more sensitive method for detecting bacterial persistence in diabetic periapical infections.
- While mortality is increased, lesion size and inflammation markers may not differ significantly in surviving diabetic mice.