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Construction and synchronization of dnaA temperature-sensitive mutants of Streptomyces
Li-Fong Lee1, Shun-Hua Yeh, Carton W Chen
1Institute of Genetics, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan.
Journal of Bacteriology
|January 25, 2002
Abstract:
Temperature-sensitive mutants of Streptomyces defective in initiation of chromosome replication were created by in vitro site-directed mutagenesis in the dnaA gene followed by gene replacement. When they were shifted to 39 degrees C replication in the mutants ceased in about 90 min but resumed on return to 30 degrees C. This allowed manipulations to achieve replication synchronization.