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Updated: Sep 26, 2026

Label-free in situ Imaging of Lignification in Plant Cell Walls
Published on: November 1, 2010
Chemical analysis of wood chips in motion using thermal-emission mid-infrared spectroscopy with projection to latent
Roger W Jones1, Robert R Meglen, Bonnie R Hames
1Ames Laboratory, Iowa State University, 50011, USA. jonesrw@ameslab.gov
Abstract:
The opacity and random surface orientation of process streams of solids complicates the application of spectroscopy to on-line analysis of solid streams. We have used a specialized form of thermal-emission mid-infrared spectroscopy, called transient infrared spectroscopy, combined with PLS regression to analyze a moving stream of wood chips for lignin, hemicellulose, glucan, and extractives content. Sixty-eight softwood samples from 6 species were analyzed using one regression, and 28 hardwood samples from 14 species were analyzed with another. Root-mean-square errors of prediction for the properties varied from 0.8 to 1.9 wt %, depending on property and wood type, with lignin and hemicellulose being the most accurately determined and glucan being the least.
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