[Assembly and evaluation of tissue engineered human cellular alveolar bony implanting materials in vitro]

To assembly human cellular alveolar bony implanting materials in vitro and evaluate their osteogenic activities.

Human alveolar bone cells were separated from alveolar bone around the third impacted teeth of 3 patients by enzyme digestion and cultured in alpha-MEM containing beta-glycerophosphate and Dexamethasone at 5% CO2, 37 degrees C for 21-28 days. Confirmed osteoblasts-like cells were then seeded onto 3 kinds of degradable biomaterials of polyglycolic acid scaffold, collagen sponge, and L-lactic acid/epsilon-caprolactone to form the cell-scaffold complexes. The 3 types of complexes were continued to culture for 21-28 days at the same conditions. The cell proliferation, morphological changes, ALPase activity and mineral nodule formation on scaffolds were measured and observed at 3 days intervals.

The results indicated that the cultured human alveolar bone origin cells from 3 patients could successfully express the osteoblasts phenotype in single layered culturing after stimulated by beta-glycerophosphate and Dexamethasone. The cultured osteoblast-like cells seeded on PGAS matrix had the highest attachment, proliferative and osteogenic activities, suggesting a good bio-affinity between the human alveolar osteoblast-like cells and the PGAS matrix. The statistical analysis (ANOVA) showed that there were significant differences between PGAS- osteoblasts complex and CLGS or LACT complexes on osteogenic activities (P < 0.05).

PGAS-osteoblast complex is worth to be further developed into a tissue-engineered cellular artificial bony implant for reconstructing the oral-maxillofacial bony defects.