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[A successive study of histopathological changes in unilateral facial muscle denervation]
1Department of Oral Maxillofacial Surgery, Peking University School of Stomatology, Beijing 100081, China.
Objective:
To observe the histopathological changes in unilateral facial muscle denervation.
Methods:
Gomori trichrome stain, acridine orange (AO) fluorescence, enzyme-histochemistry and immunohistochemistry techniques were used for studying mitochondria, nuclei acid concentration, enzymatic activity and contraction protein expression of facial muscle in different denervation period.
Results:
Mitochondrian function, enzymatic activity and contraction protein expression of facial muscle denervation increased in the first two weeks, then declined till 6 months. Myofiber's histotype began to transform in 1 month after denervation. Type-grouping was obvious in 6 month. Regeneration myofibers were also seen at this time.
Conclusions:
Unilateral facial muscles become atrophy after denervation. They have proliferating tendency from 2 month to 6 month. Therefore, within 1 month after denervation measures to benefit atrophy will be more effective.