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Determination of biogenic amines in wine by multidimensional liquid chromatography with online derivatisation
T Hyötyläinen1, N Savola, P Lehtonen
1Department of Chemistry, University of Helsinki, Finland.
Abstract:
An online multidimensional liquid chromatographic system was developed for online clean-up, derivatisation and separation of biogenic amines in wines. The system consists of a cation-exchange precolumn, a derivatisation coil and an analytical column, and a column-switching valve. The entire system can be easily automated. The method proved to be quantitative and sensitive. Limits of detection were below 0.05 mg l(-1) for all amines and linearity was preserved over the tested range (0.05-15 mg l(-1)). The method was applied to the analysis of red wines of different origin.
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