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An anhydrous polymorphic form of trehalose.

Hiromasa Nagase1, Tomohiro Endo, Haruhisa Ueda

  • 1Department of Physical Chemistry, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan. nagase@hoshi.ac.jp

Carbohydrate Research
|January 30, 2002
PubMed
Summary

An anhydrous form of alpha,alpha-trehalose was created. This stable, hygroscopic form rapidly reverts to dihydrate at high humidity, potentially explaining trehalose

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Area of Science:

  • Biochemistry
  • Materials Science

Background:

  • Trehalose is a disaccharide known for its protective properties in biological systems.
  • Understanding the different polymorphic forms of trehalose is crucial for its applications.

Purpose of the Study:

  • To prepare and characterize an anhydrous polymorphic form of alpha,alpha-trehalose.
  • To investigate the stability and hygroscopic properties of this anhydrous form.

Main Methods:

  • Preparation of anhydrous trehalose from trehalose dihydrate using vacuum heating and hot air drying.
  • Characterization using X-ray powder diffraction and differential scanning calorimetry.
  • Assessment of hygroscopicity and phase transitions at varying relative humidity and temperature.

Main Results:

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  • An anhydrous polymorphic form of alpha,alpha-trehalose was successfully prepared, with vacuum heating yielding reproducible results.
  • The anhydrous form is hygroscopic, converting back to trehalose dihydrate at 43% relative humidity and 25 degrees C.
  • The anhydrous form transforms into an amorphous phase at 127 degrees C, which remained unchanged under tested humidity conditions.

Conclusions:

  • The prepared anhydrous trehalose exhibits distinct phase transition and hygroscopic behaviors.
  • These properties may contribute to the role of trehalose in dehydration tolerance observed in desert plants and insects.
  • Further research into trehalose polymorphism can inform its use in stabilizing biological materials.