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[Determination of azoxystrobin in tea by HPLC]
1Hokkaido Institute of Public Health: Nishi 12-chome, Kita 19-jo, Kita-ku, Sapporo 060-0819, Japan.
Summary
A new HPLC method accurately determines azoxystrobin in tea. This method efficiently removes interfering compounds, ensuring reliable quantification of the fungicide residue.
Area of Science:
- Agricultural Chemistry
- Analytical Chemistry
Background:
- Azoxystrobin is a widely used fungicide in tea cultivation.
- Accurate determination of pesticide residues is crucial for food safety and regulatory compliance.
Purpose of the Study:
- To develop and validate a High-Performance Liquid Chromatography (HPLC) method for the determination of azoxystrobin in tea samples.
Main Methods:
- Extraction of azoxystrobin using acetone.
- Clean-up procedures involving alumina, Sep-Pak Vac tC18, liquid-liquid partition, Florisil, and silica-gel columns.
- HPLC analysis using a C18 column with acetonitrile-water mobile phase and UV detection at 260 nm.
Main Results:
- The method achieved a recovery rate of 90.2% for azoxystrobin fortified at 0.4 µg/g.
- The limit of determination was established at 0.2 µg/g, indicating high sensitivity.
Conclusions:
- The developed HPLC method is suitable for the accurate and reliable determination of azoxystrobin residues in tea.
- This method provides a valuable tool for monitoring azoxystrobin levels in tea products.