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Updated: Jul 28, 2026
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Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Enantiomer separation by capillary electrochromatography using fritless packed columns
1Department of Chemistry, Hamamatsu University School of Medicine, Japan. fujimoto@hama-med.ac.jp
Summary
This review covers advances in separating enantiomers using capillary electrochromatography with fritless packed columns. These columns are prepared using sintering, sol-gel methods, or in situ polymerization for effective chiral separations.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Enantiomer separation is crucial in pharmaceuticals and chemical synthesis.
- Capillary electrochromatography (CEC) offers high separation efficiency.
- Fritless packed columns enhance performance in CEC.
Purpose of the Study:
- To review recent developments in enantiomer separation using fritless packed columns in CEC.
- To detail column preparation methods and performance.
Main Methods:
- Preparation of fritless packed columns in fused silica capillaries.
- Immobilization of chiral packing materials via sintering or sol-gel technology.
- In situ polymerization of chiral selectors within the capillary.
Main Results:
- Successful separation of various enantiomers using the described fritless packed columns.
- Demonstration of different column preparation techniques and their impact on performance.
- Attainable column performance metrics are detailed.
Conclusions:
- Fritless packed columns are effective for enantiomer separation in CEC.
- Sintering, sol-gel, and in situ polymerization are viable preparation methods.
- This review provides insights into column preparation and performance for chiral separations.
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