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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: Jul 19, 2026

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

Gene expression profiling of lymphoid malignancies.

Louis M Staudt¹

¹Metabolism Branch, Center for Cancer Research, National Cancer Institute, 9000 Rockville Pike, Bethesda, Maryland 20892, USA. lstaudt@mail.nih.gov

Annual Review of Medicine

|January 31, 2002

Summary

Gene expression profiling classifies cancers, revealing diffuse large B cell lymphoma (DLBCL) as two distinct diseases and chronic lymphocytic leukemia (CLL) as one, with subtypes impacting treatment.

Area of Science:

Oncology
Molecular Biology
Genomics

Background:

Gene expression profiling offers molecular classification for cancer, enabling homogeneous categorization based on pathogenesis and clinical behavior.
DNA microarrays facilitate comprehensive gene expression analysis, crucial for understanding cancer heterogeneity.

Purpose of the Study:

To classify cancers at a molecular level using gene expression profiling.
To investigate the molecular subtypes and pathogenesis of diffuse large B cell lymphoma (DLBCL) and chronic lymphocytic leukemia (CLL).

Main Methods:

Utilized DNA microarrays for comprehensive gene expression profiling.
Analyzed gene expression signatures to differentiate cancer subtypes and identify molecular mechanisms.

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Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

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Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

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Last Updated: Jul 19, 2026

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Published on: January 19, 2017

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

Main Results:

Diffuse large B cell lymphoma (DLBCL) comprises at least two distinct molecular subtypes with different B cell differentiation origins and clinical outcomes.
Chronic lymphocytic leukemia (CLL) presents as a single disease but exhibits two clinically divergent subtypes, with B cell antigen receptor signaling implicated in aggressive forms.
Gene expression analysis elucidated lymphomagenesis from BCL-6 translocations, identifying the NF-kappa B pathway as a therapeutic target in DLBCL.

Conclusions:

Gene expression profiling is a powerful tool for molecular cancer classification and understanding disease heterogeneity.
Distinct molecular subtypes within DLBCL and CLL necessitate tailored therapeutic strategies.
The NF-kappa B signaling pathway represents a promising therapeutic target for DLBCL.