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Studies on the flow and distribution of leukocytes in mesentery microcirculation of rats
Abstract:
AIM:To study the effect of leukocyte-endothelium interaction (LEI) on the flow and distribution of leukocytes in microcirculation under physiological condition.METHODS:A microcirculation image multiple parameter computer analysis system (MIMPCAS) was used to study the flow and distribution of leukocytes in mesentery microcirculation of rats in vivo.RESULTS:The difference of visible leukocyte flux (VLF) was as high as 131 times in the arterioles and venules with similar diameter and blood velocity. The visible leukocytes rolled along the blood vessel wall as a jerky movement. The frequency distribution of the visible leukocyte velocity (VLV) showed a two peak curve. The low peak value was on 10&mgr;m/s-15&mgr;m/s while the high peak fell between 25&mgr;m/s-30&mgr;m/s. With the increase of diameter of venules, VLF increased while the VLV remained at the same level. With the increase of RBC velocity, VLV trends to elevate and VLF to fall down.CONCLUSION:The results herein might provide a basic theory for the study on the mechanism of LEI under physiological condition and novel methods for the prevention and treatment of high LEI in many pathological processes.
Insights
Leukocyte-endothelium interaction (LEI) significantly impacts leukocyte flow and distribution in microcirculation. Understanding these dynamics offers new strategies for preventing and treating conditions linked to excessive LEI.
Area of Science:
- Physiology
- Microcirculation Biology
- Immunology
Background:
- Leukocyte-endothelium interaction (LEI) is crucial in microcirculation.
- Understanding LEI under physiological conditions is essential for disease research.
Purpose of the Study:
- To investigate the effect of leukocyte-endothelium interaction (LEI) on leukocyte flow and distribution in microcirculation.
- To analyze leukocyte behavior in rat mesentery microcirculation in vivo.
Main Methods:
- Utilized a microcirculation image multiple parameter computer analysis system (MIMPCAS).
- Analyzed visible leukocyte flux (VLF) and visible leukocyte velocity (VLV) in rat mesentery.
- Correlated VLF and VLV with vessel diameter and red blood cell (RBC) velocity.
Main Results:
- Visible leukocyte flux (VLF) varied significantly (up to 131 times) between vessels of similar size and blood velocity.
- Visible leukocytes exhibited jerky rolling movements along the vessel wall.
- Leukocyte velocity distribution showed a bimodal curve with peaks at 10–15 µm/s and 25–30 µm/s.
- VLF increased with venule diameter, while VLV remained constant.
- Elevated RBC velocity led to increased VLV and decreased VLF.
Conclusions:
- Findings provide foundational insights into LEI mechanisms under physiological conditions.
- The study introduces novel methods for preventing and treating conditions associated with high LEI.