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Updated: Oct 2, 2026

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
Published on: October 20, 2021
Identification of the epitopes on HCV core protein recognized by HLA-A2 restricted cytotoxic T lymphocytes
1Department of Immunology, the Fourth Military Medical University, Xi'an 710033, Shaanxi Province, China. juliu@fmmu.edu.cn
Aim:
To identify hepatitis C virus(HCV) core protein epitopes recognized by HLA-A2 restricted cytotoxic T lymphocyte (CTL).
Methods:
Utilizing the method of computer prediction followed by a 4h(51)Cr release assay confirmation.
Results:
The results showed that peripheral blood mononuclear cells (PBMC) obtained from two HLA-A2 positive donors who were infected with HCV could lyse autologous target cells labeled with peptide "ALAHGVRAL (core 150-158)". The rates of specific lysis of the cells from the two donors were 37.5% and 15.8%, respectively. Blocking of the CTL response with anti-CD4 mAb caused no significant decrease of the specific lysis. But blocking of CTL response with anti-CD8 mAb could abolish the lysis.
Conclusion:
The peptide (core 150-158) is the candidate epitope recognized by HLAA2 restricted CTL.
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