Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Platinum complexes of nucleotides.

P C Kong, T Theophanides

    Bioinorganic Chemistry
    |January 1, 1975
    PubMed
    Summary

    Platinum(II) complexes react with nucleotides like CMP and GMP at specific nitrogen sites, not the phosphate group. AMP binds to platinum at N1 and/or N7 sites, requiring a higher platinum to nucleotide ratio.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The effects of metal ion contaminants on the double stranded DNA helix and diseases.

    Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering·2017
    Same author

    Copper and carcinogenesis.

    Critical reviews in oncology/hematology·2002
    Same author

    Magnesium-DNA interactions and the possible relation of magnesium to carcinogenesis. Irradiation and free radicals.

    Critical reviews in oncology/hematology·2002
    Same author

    Natural abundance (15)N CP MAS NMR as a novel tool for investigating metal binding to nucleotides in the solid state.

    Inorganic chemistry·2001
    Same author

    Syntheses of new ionic technetium(III) complexes containing four monodentate phosphine ligands. Crystal structures of trans-[Tc(PMe2Ph)4Cl2]PF6, trans-[Tc(PMe3)4Cl2]BPh4, and trans-[Tc(PMe3)4Cl2]PF6.

    Inorganic chemistry·2001
    Same author

    The role of free radical reactions with haemoglobin and thalassaemia.

    Journal of inorganic biochemistry·2000

    Area of Science:

    • Inorganic Chemistry
    • Bioinorganic Chemistry
    • Medicinal Chemistry

    Background:

    • Platinum(II) complexes are investigated for their potential anticancer properties.
    • Nucleotides are fundamental building blocks of nucleic acids and play crucial roles in cellular processes.
    • Understanding the coordination chemistry of platinum complexes with nucleotides is essential for designing targeted therapies.

    Purpose of the Study:

    • To elucidate the coordination modes of platinum(II) complexes with cytidine 5'-monophosphate (CMP), guanosine 5'-monophosphate (GMP), and adenosine 5'-monophosphate (AMP).
    • To determine the binding sites and stoichiometry of the reactions between [Pt(dien)Cl2] and these nucleotides.
    • To investigate the influence of nucleotide structure on platinum coordination.

    Main Methods:

    • Nuclear magnetic resonance (NMR) spectroscopy was employed to study the reaction products.
    • Stoichiometric studies were conducted to determine the optimal ratios for platinum-nucleotide complex formation.

    Main Results:

    • CMP coordinates to the platinum atom via the N3 site.
    • GMP coordinates to the platinum atom via the N7 site, with no additional binding sites observed even in excess platinum.
    • AMP coordinates to the platinum at the N1 and/or N7 sites, with a complete reaction observed at a 4:1 platinum to AMP ratio.
    • Phosphate group binding to platinum was not detected for any of the studied nucleotides.

    Conclusions:

    • The coordination of [Pt(dien)Cl2] to CMP, GMP, and AMP occurs through specific nitrogen atoms within the nucleobases.
    • The binding preferences and stoichiometry vary depending on the specific nucleotide, highlighting the selectivity of platinum coordination.
    • These findings contribute to the understanding of platinum-nucleotide interactions, relevant for developing platinum-based chemotherapeutics.

    Related Experiment Videos