Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A cyclophilin functions in pre-mRNA splicing.

David S Horowitz1, Edward J Lee, Stephen A Mabon

  • 1Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. dhorowitz@usuhs.mil

The EMBO Journal
|February 2, 2002
PubMed
Summary

Cyclophilins, like USA-CyP, are crucial for pre-mRNA splicing by forming complexes with spliceosomal proteins hPrp18 and hPrp4. The drug cyclosporin A inhibits splicing by blocking USA-Cyclophilin

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome reorganization and its functional impact during breast cancer progression.

eLife·2026
Same author

Connecting multiway enhancer-promoter interactions to changes in gene expression in cancer.

bioRxiv : the preprint server for biology·2026
Same author

Orderly mitosis shapes interphase genome architecture.

eLife·2026
Same author

TAD boundaries and gene activity are uncoupled.

eLife·2026
Same author

Acute degron-mediated RUNX1 loss reprograms enhancer activity to epigenetically drive epithelial destabilization and initiate cancer hallmarks.

bioRxiv : the preprint server for biology·2026
Same author

Protocol to measure transcriptional bursting of endogenous genes using high-throughput RNA-FISH.

STAR protocols·2026

Area of Science:

  • Molecular Biology
  • RNA Splicing
  • Protein-Protein Interactions

Background:

  • Pre-mRNA splicing is a fundamental process in gene expression.
  • Cyclophilins are a class of proteins involved in various cellular functions.
  • The role of cyclophilins in the spliceosome remains incompletely understood.

Purpose of the Study:

  • To investigate the role of cyclophilin USA-CyP in pre-mRNA splicing.
  • To elucidate the interaction of USA-CyP with spliceosomal proteins.
  • To determine the mechanism by which cyclosporin A affects splicing.

Main Methods:

  • Co-immunoprecipitation to identify protein complexes.
  • In vitro splicing assays to assess functional impact.
  • In vivo splicing assays to confirm cellular effects.

Related Experiment Videos

Main Results:

  • USA-Cyclophilin (USA-CyP) forms stable complexes with spliceosomal proteins hPrp18 and hPrp4.
  • The drug cyclosporin A inhibits pre-mRNA splicing by blocking USA-CyP's function within the hPrp18 complex.
  • USA-CyP is proposed to be recruited to the spliceosome via these complexes, acting during splicing.

Conclusions:

  • USA-CyP plays a direct role in both steps of pre-mRNA splicing.
  • Cyclophilins can function within large molecular machines like the spliceosome.
  • These findings offer insights into cyclophilin mechanisms and drug action.