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[PCR in detecting the correlation between infection of HBV and cholelithiasis]
1Department of Surgery, First Clinical Hospital, China Medical University, Shenyang 110001.
Insights
Hepatitis B virus (HBV) infection is correlated with gallstone formation. HBV-DNA was detected in 40.63% of gallstone patients, significantly higher than in non-gallstone patients.
Area of Science:
- Hepatology and Gastroenterology
- Viral Infections and Immunology
Context:
- Cholelithiasis, or gallstones, affects a significant portion of the global population.
- Hepatitis B virus (HBV) is a major cause of chronic liver disease worldwide.
- The potential link between HBV infection and gallstone development requires further investigation.
Purpose:
- To investigate the correlation between Hepatitis B virus (HBV) infection and the presence of cholelithiasis.
- To determine the prevalence of HBV-DNA in gallbladder tissues of patients with and without gallstones.
Summary:
- A polymerase chain reaction-Ethidium bromide (PCR-EB) assay was performed on 52 gallbladder samples from patients positive for HBV serologic markers.
- HBV-DNA was identified in 40.63% of cholelithiasis patients (13 out of 32) compared to 15% of non-cholelithiasis patients (3 out of 20).
- These findings indicate a statistically significant association between HBV infection and gallstone formation.
Impact:
- This study suggests a potential role of HBV in the pathogenesis of cholelithiasis.
- Understanding this correlation may inform preventative strategies and clinical management for patients with HBV.
- Further research into the mechanisms underlying this association is warranted.
Objective:
To study the correlation between cholelithiasis and the infection of HBV.
Method:
32 formalin-fixed and paraffin-embedded gallbladder samples of cholelithiasis patients and 20 gallbladder samples of non-cholelithiasis patients were investigated using the polymerase chain reaction-Ethidium bromide (PCR-EB) assay. The 52 patients were positive to HBV serologic markers.
Result:
The results showed that HBV-DNA was found in 13 gallbladder samples of 32 cholelithiasis patients (40.63%), significantly higher than that in 3 gallbladder samples of 20 non-cholelithiasis patients (15%).
Conclusion:
The infection of HBV and the formation of cholelithiasis are correlated.