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[Culture of chondrocytes seeded onto chitin scaffoldings coating with different materials: experimental study]
1Department of Plastic Surgery, Shanghai Ninth Hospital, Shanghai Second Medical University, Shanghai 200011.
Objective:
To find the possibility of chitin used as scaffolds in tissue engineering.
Method:
Chondrocytes were cultured when they were seeded onto chitin meshcoated with polylactic acid (PLA) lecithin (LEC) and poly-l-lysine (PLYS). With light microscope and scanning electron microscope, the scaffolds in hydrophilia and absorptivity to chondrocytes and the function of the cells were observed.
Result:
LEC can change the hydrophilia of scaffolds. PLYS can strengthen the absorptivity of the scaffolds. and promote the matrices production of chondrocytes.
Conclusion:
The chitin coated with LEC and PLYS can be used as scaffolds in tissue engineering.