Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Freely programmable automatic radioimmunoanalysis on the STRATEC ST 300 analyzer].

J Uhrová1, P Stern, T Zima

  • 1Institute of Clinical Biochemistry, General University Hospital, Karlovo nám. 32, 121 11 Prague 2, Czech Republic. uhrova@vfn.cz

Sbornik Lekarsky
|February 8, 2002
PubMed
Summary

Automatic radioimmunoassay (RIA) on the STRATEC SR 300 analyser is significantly faster than manual methods for large sample sets. Both isotope and non-isotope immunoassays show comparable results, with RIA offering cost advantages.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circulating Cell-Free DNA Extraction from Liquid Biopsy for Cancer Research.

Folia biologica·2023
Same author

Prognostic factors of renal cell carcinoma.

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2022
Same author

Time and Temperature Stability of TGF-β1, EGF and IGF-1 in 20% and 100% Human Serum.

Folia biologica·2022
Same author

Pancreatic stellate cells - rising stars in pancreatic pathologies.

Physiological research·2022
Same author

The Effects of Different Storage Conditions and Repeated Freeze/Thaw Cycles on the Concentration, Purity and Integrity of Genomic DNA.

Folia biologica·2021
Same author

The effect of surgery on the levels of matrix metalloproteinases in patients with inguinal hernia.

Physiological research·2021

Area of Science:

  • Clinical Chemistry
  • Immunoassay Technology

Context:

  • Radioimmunoassay (RIA) and Immunoradiometric Assay (IRMA) are established immunoassay techniques.
  • Automation in laboratory diagnostics is increasingly important for efficiency and throughput.

Purpose:

  • To compare the performance of a free programmable automatic RIA analyser (STRATEC SR 300) with manual RIA and IRMA methods.
  • To evaluate the impact of sample volume, reagent delivery, and washing steps on analysis speed.
  • To assess qualitative and quantitative differences between isotope and non-isotope immunoassays.

Summary:

  • Manual delivery was faster for individual steps, but automatic analysis proved significantly faster overall for a set of 100 analyses (20-90 minutes saved).
  • Dispensing speed is influenced by sample/reagent volumes and rinsing/washing parameters.

Related Experiment Videos

  • No significant qualitative or quantitative differences were observed between isotope and non-isotope immunoassays.
  • Radioimmunoassays offer cost advantages and a wide selection of analytical kits, independent of user instrumentation.

    • Impact:

    • Automation of RIA enhances laboratory efficiency, particularly for high-throughput testing.
    • Cost-effectiveness and assay availability favor RIA for routine diagnostics.
    • Non-isotope methods may offer faster turnaround times for small series or individual tests.