Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neurotrauma and its mortality.

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2026
Same author

A rare case of mediastinal pseudocyst in patient after acute pancreatitis.

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2025
Same author

Prognostic factors of renal cell carcinoma.

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2022
Same author

Time and Temperature Stability of TGF-β1, EGF and IGF-1 in 20% and 100% Human Serum.

Folia biologica·2022
Same author

Diagnosis and treatment of surgical skin and soft tissue infections - current status.

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2022
Same author

Microbiological analysis of peritoneal fluid samples from patients with perforated peptic ulcer - retrospective observational study.

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2022

Related Experiment Video

Updated: Aug 8, 2025

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
05:26

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

Published on: September 16, 2022

4.0K

Circulating Cell-Free DNA Extraction from Liquid Biopsy for Cancer Research.

L Pfeiferova1, M Safarikova1, J Ulrych2

  • 1Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic.

Folia Biologica
|March 5, 2023
PubMed
Summary

Manual plasma DNA extraction yielded more circulating tumor DNA with fewer contaminants than automated methods. This supports liquid biopsy

More Related Videos

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
08:25

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Published on: September 9, 2020

11.2K
Automatic Separation and Collection of Cancer-Related Substances from Clinical Samples
08:49

Automatic Separation and Collection of Cancer-Related Substances from Clinical Samples

Published on: January 13, 2023

1.9K

Related Experiment Videos

Last Updated: Aug 8, 2025

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
05:26

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

Published on: September 16, 2022

4.0K
Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
08:25

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Published on: September 9, 2020

11.2K
Automatic Separation and Collection of Cancer-Related Substances from Clinical Samples
08:49

Automatic Separation and Collection of Cancer-Related Substances from Clinical Samples

Published on: January 13, 2023

1.9K

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Global cancer incidence is rising, increasing the demand for reliable biomarkers.
  • Tissue biopsy limitations include invasiveness and difficulty in sample acquisition.
  • Liquid biopsy offers a less invasive alternative for cancer diagnosis, prognosis, and monitoring.

Purpose of the Study:

  • To compare the efficacy of two circulating cell-free DNA isolation methods from plasma.
  • To evaluate manual versus automated techniques for DNA yield and purity.

Main Methods:

  • Isolation of circulating cell-free DNA from plasma using the manual QIAamp Circulating Nucleic Acid Kit.
  • Isolation of circulating cell-free DNA from plasma using the automated MagNA Pure Compact (MPC) system.
  • Quantification and purity assessment of extracted DNA.

Main Results:

  • Manual extraction yielded significantly higher amounts of circulating tumor DNA (P < 0.05).
  • DNA isolated manually exhibited lower levels of organic and protein contaminants.
  • Both methods produced DNA suitable for downstream molecular genetic analyses.

Conclusions:

  • Manual QIAamp Circulating Nucleic Acid Kit provides superior yield and purity for circulating tumor DNA isolation from plasma.
  • Liquid biopsy using cell-free DNA is a promising alternative to tissue biopsy.
  • Further optimization of automated methods may be needed to match manual extraction performance.