Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nef and LTR sequence variation from sequentially derived human immunodeficiency virus type 1 isolates.

T McNearney1, Z Hornickova, A Templeton

  • 1Department of Medicine,Washington University, St Louis, Missouri 63110, USA.

Virology
|April 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vitro developmental competence of bovine demi-embryos generated by blastomere separation and blastocyst bisection.

Reproduction in domestic animals = Zuchthygiene·2024
Same author

Single-Cell Transcriptomic Analysis of Kaposi Sarcoma.

bioRxiv : the preprint server for biology·2024
Same author

97&#x2003;Generation of <i>GGTA1</i> knockout porcine blastocysts by intracytoplasmic sperm injection mediated gene edition.

Reproduction, fertility, and development·2022
Same author

74&#x2003;Gene expression analysis of developmental key genes in <i>in vitro</i> bovine twin embryos produced by blastomeres separation and embryo bisection.

Reproduction, fertility, and development·2022
Same author

How distant? An experimental analysis of students' COVID-19 exposure and physical distancing in university buildings.

International journal of disaster risk reduction : IJDRR·2022
Same author

A delayed dose of quadrivalent human papillomavirus vaccine demonstrates immune memory in HIV-1-infected men.

Papillomavirus research (Amsterdam, Netherlands)·2018
Same journal

Regulation of HIV-2 Vpx/Vpr expression by the poly-proline motif: Enhancement of protein dimerization and translation.

Virology·2026
Same journal

Distinct virus-specific regulation of RNA synthesis across genome segments by thogotovirus polymerases: insights from Oz virus and Dhori virus.

Virology·2026
Same journal

Viral diversity in predatory insects used for biological control: Insights from a multi-country meta-viromic analysis.

Virology·2026
Same journal

Unveiling the hidden virome of Sclerotinia sclerotiorum: New tools and discoveries in mycovirus detection.

Virology·2026
Same journal

ASFV pDP238L negatively regulates type I interferon production via inhibiting the methylation of TBK1.

Virology·2026
Same journal

Divergent poxvirus identified in a non-native black rat from Madagascar.

Virology·2026
See all related articles

HIV-1 sequence diversity increases with disease progression. Nef-deleted strains replicate in vivo, suggesting viral adaptation during infection.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Human Immunodeficiency Virus type 1 (HIV-1) infection is characterized by viral evolution.
  • The nef gene and Long Terminal Repeat (LTR) regions are critical for HIV-1 replication and pathogenesis.
  • Understanding viral sequence variation during disease progression is crucial for therapeutic strategies.

Purpose of the Study:

  • To longitudinally assess DNA sequence variation in HIV-1 nef and LTR.
  • To analyze the impact of disease progression on viral quasispecies diversity.
  • To investigate the replication capacity of nef-deleted HIV-1 variants in vivo.

Main Methods:

  • Longitudinal analysis of HIV-1 nef and LTR DNA sequences from four infected subjects.
  • Quasispecies analysis at single time points during disease progression.

Related Experiment Videos

  • Identification and characterization of point mutations, deletions, and rearrangements.

    • Main Results:

    • Point mutations were observed within quasispecies at individual time points.
    • Viral diversity increased with disease progression in most analyzed patients.
    • Deletions and rearrangements in viral sequences were more prevalent in later disease stages.
    • Nef-deleted HIV-1 quasispecies demonstrated in vivo replication, coexisting with nef-bearing variants.

    Conclusions:

    • HIV-1 quasispecies exhibit significant sequence evolution, including deletions, during disease progression.
    • Nef-deleted HIV-1 variants are replication-competent in vivo.
    • The coexistence of nef-deleted and nef-bearing quasispecies suggests complex viral adaptation mechanisms and potential complementation.