Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

PTH and DA regulate Na-K ATPase through divergent pathways.

Syed Jalal Khundmiri1, Eleanor Lederer

  • 1Veterans Administration and Department of Medicine, University of Louisville, School of Medicine, Louisville, Kentucky 40202, USA.

American Journal of Physiology. Renal Physiology
|February 8, 2002
PubMed
Summary

Parathyroid hormone (PTH) and dopamine (DA) differentially regulate Na-K ATPase in kidney cells. While both inhibit activity, they utilize distinct signaling pathways and cellular mechanisms for this regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phosphate homeostasis and apparent treatment-resistant hypertension in chronic kidney disease: the Chronic Renal Insufficiency Cohort (CRIC) study.

Hypertension research : official journal of the Japanese Society of Hypertension·2026
Same author

Leveraging quantitative systems pharmacology and artificial intelligence to advance treatment of chronic kidney disease mineral bone disorder.

American journal of physiology. Renal physiology·2024
Same author

Understanding renal phosphate handling: unfinished business.

Current opinion in nephrology and hypertension·2023
Same author

Preclinical and Clinical Evidence of Effect of Acid on Bone Health.

Advances in chronic kidney disease·2022
Same author

Na/H Exchange Regulatory Factor 1 Deficient Mice Show Evidence of Oxidative Stress and Altered Cisplatin Pharmacokinetics.

Antioxidants (Basel, Switzerland)·2021
Same author

Current State of the Workforce in Nephrology.

Advances in chronic kidney disease·2020

Area of Science:

  • Nephrology
  • Cellular Physiology
  • Molecular Biology

Background:

  • Parathyroid hormone (PTH) and dopamine (DA) are known to inhibit sodium-phosphate cotransport in proximal tubular cells.
  • Previous research indicated that PTH and DA utilize different signaling pathways to inhibit phosphate transport.
  • This suggests that their effects on other transporters, like Na-K ATPase, might also be mediated by divergent mechanisms.

Purpose of the Study:

  • To investigate whether parathyroid hormone (PTH) and dopamine (DA) inhibit Na-K ATPase activity through distinct signaling pathways.
  • To elucidate the specific signaling cascades involved in the early and late phases of Na-K ATPase inhibition by PTH and DA.

Main Methods:

  • Opossum kidney (OK) cells were used to measure Na-K ATPase activity.

Related Experiment Videos

  • Inhibition of Na-K ATPase by PTH and DA was assessed in the presence of various signaling pathway inhibitors.
  • Extracellular signal-regulated kinase (ERK) activation and pertussis toxin sensitivity were evaluated.
  • Basolateral membrane expression of the Na-K ATPase alpha-subunit was analyzed.

    • Main Results:

    • Both PTH and DA exhibited biphasic inhibition of Na-K ATPase activity.
    • Early inhibition involved protein kinase C (PKC) and phospholipase A(2) (PLA(2)) pathways, while late inhibition involved protein kinase A and PLA(2).
    • ERK inhibition blocked PTH's effect but not DA's, whereas pertussis toxin blocked DA's effect but not PTH's.
    • Dopamine, but not PTH, caused early downregulation of the alpha-subunit's basolateral membrane expression.

    Conclusions:

    • Parathyroid hormone (PTH) and dopamine (DA) regulate Na-K ATPase activity through distinct signaling mechanisms.
    • PTH primarily utilizes PKC-, PLA(2)-, and ERK-dependent pathways, while DA involves pertussis toxin-sensitive pathways.
    • These findings highlight divergent cellular strategies employed by PTH and DA to modulate kidney proximal tubule function.