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Related Experiment Videos

p63 expression profiles in human normal and tumor tissues.

Charles J Di Como1, Marshall J Urist, Irina Babayan

  • 1Division of Molecular Pathology, Department of Pathology, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
|February 13, 2002
PubMed
Summary

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The p63 gene is crucial for development and is expressed in epithelial cells. Its expression is linked to squamous cell carcinomas, transitional cell carcinomas, lymphomas, and thymomas, but not adenocarcinomas.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The p63 gene, a member of the p53 family, is located on chromosome 3q27-28.
  • The p63 gene product is essential for normal development.

Purpose of the Study:

  • To investigate the expression pattern of the p63 gene in various human normal and tumor tissues.
  • To determine the association of p63 expression with specific cancer types.

Main Methods:

  • Immunohistochemistry using a monoclonal antibody (clone 4A4) recognizing all p63 splice variants.
  • Reverse transcription-polymerase chain reaction (RT-PCR) with isoform-specific primers.

Main Results:

  • p63 expression is nuclear, localized to nucleoplasmic patterns.

Related Experiment Videos

  • p63 is expressed in epithelial cells of stratified epithelia (skin, esophagus, bladder, etc.) and basal cells in glandular structures (prostate, breast, bronchi).
  • p63 is predominantly expressed in basal cell, squamous cell, and transitional cell carcinomas, as well as thymomas and some non-Hodgkin's lymphomas; it is not detected in adenocarcinomas, endocrine tumors, germ cell neoplasms, melanomas, or soft tissue sarcomas.

    • Conclusions:

    • p63 plays a role in the development of squamous cell carcinomas, transitional cell carcinomas, certain lymphomas, and thymomas.
    • The expression profile of p63 suggests its involvement in specific epithelial and lymphoid malignancies.