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Updated: Oct 2, 2026

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Published on: December 31, 2019
Membrane anchors for vacuolar targeting: application in plant bioreactors
1Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China. ljiang@cuhk.edu.hk
Abstract:
Transgenic plants are attractive expression systems for producing recombinant proteins. Plant cells compartmentalize and store metabolites and proteins in vacuoles, but foreign proteins need to be targeted to the correct compartments if they are to accumulate in a stable fashion. Here we present a general strategy in which unique transmembrane and cytoplasmic tail sequences are used as anchors for delivering recombinant proteins via distinct vesicular transport pathways to specific vacuolar compartments where stable accumulation can occur.
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