Related Experiment Video

Updated: Aug 8, 2026

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
09:28

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Published on: June 25, 2010

Automated high-resolution gas chromatographic system for recording and evaluation of metabolic profiles

M L McConnell, M Novotný

    Journal of Chromatography
    |October 29, 1975
    PubMed

    More Related Videos

    Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics07:21

    Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics

    Published on: June 6, 2018

    Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems14:42

    Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

    Published on: September 23, 2021

    Related Concept Videos

    Gas Chromatography–Mass Spectrometry (GC–MS)01:14

    Gas Chromatography–Mass Spectrometry (GC–MS)

    Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
    A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...
    Mass Spectrometry: Complex Analysis01:21

    Mass Spectrometry: Complex Analysis

    Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
    GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
    Gas Chromatography: Introduction01:13

    Gas Chromatography: Introduction

    Gas chromatography (GC) is a technique for separating and analyzing volatile compounds in a sample. Its primary purpose is to identify and quantify components in complex mixtures, making it essential in fields such as environmental analysis, pharmaceuticals, and petrochemicals. GC is also called vapor-phase chromatography (VPC) or gas-liquid partition chromatography (GLPC).
    In GC,  a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a column.
    Gas Chromatography: Sample Injection Systems01:08

    Gas Chromatography: Sample Injection Systems

    In gas chromatography, the sample is introduced as a vapor plug into the carrier gas stream for high efficiency and resolution. A microsyringe injects the sample solution into a heated sample port, vaporizing it and mixing it with the carrier gas. This process is important to ensure the sample is properly prepared for analysis. Thermally sensitive samples can be injected directly into the column and volatilized by slowly increasing the column temperature.
    Two primary injection methods are used...
    Gas Chromatography: Overview of Detectors01:13

    Gas Chromatography: Overview of Detectors

    Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, which are displayed on a chromatogram. Detectors can be categorized into two main types: destructive and non-destructive.
    A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
    Gas Chromatography: Types of Detectors-II01:19

    Gas Chromatography: Types of Detectors-II

    In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...