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Related Experiment Videos

Elastase-induced saccular aneurysms in rabbits: a dose-escalation study.

David F Kallmes1, Naomi H Fujiwara, Stuart S Berr

  • 1Department of Radiology, University of Virginia Health Services, Charlottesville, VA 22908, USA.

AJNR. American Journal of Neuroradiology
|February 16, 2002
PubMed
Summary

Increased elastase doses did not significantly enlarge rabbit aneurysms or parent arteries. This study demonstrates that 25% elastase for 10 minutes is sufficient for creating reproducible rabbit aneurysms, minimizing adjacent artery dilation.

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Area of Science:

  • Vascular biology
  • Animal models of disease
  • Aneurysm research

Background:

  • Reproducible animal models are crucial for evaluating aneurysm therapies.
  • This study investigates the impact of elastase concentration and incubation time on aneurysm and parent artery size.

Purpose of the Study:

  • To determine if higher elastase doses lead to larger aneurysms and parent arteries in a rabbit model.
  • To establish optimal parameters for creating reproducible aneurysms for preclinical research.

Main Methods:

  • Rabbit common carotid artery aneurysms were induced using elastase incubation.
  • Varying concentrations (25%, 50%) and durations (10, 20 minutes) of elastase were tested.
  • Aneurysm and parent artery dimensions were measured via angiography after 14 days.

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Main Results:

  • All elastase-treated subjects developed enlarged arterial segments compared to controls.
  • Aneurysm dimensions (width and height) were similar across different elastase doses and durations.
  • Parent artery diameters showed a trend towards enlargement with higher elastase doses (P =.07).

Conclusions:

  • Aneurysms were reliably created in rabbits, mimicking human intracranial aneurysm sizes.
  • Elastase concentration and incubation time did not significantly influence the final aneurysm size.
  • A 25% elastase concentration for a 10-minute incubation is effective for creating rabbit aneurysms while limiting parent artery dilation.